Spermatogenic Stem Cell System in the Mouse Testis

[Spermatogenic stem cell system in the mouse testis].

Mouse spermatogenesis represents a highly potent and robust stem cell system. Decades of research have made it one of the most intensively studied mammalian tissue stem cell systems. These studies include detailed morphological examinations, posttransplantation colony formation, and in vitro culture of the stem cells; however, the nature of the stem cells as well as their niche are mostly to be...

Age-dependent expression of the cystatin-related epididymal spermatogenic (Cres) gene in mouse testis and epididymis.

AIM To investigate the spatial and temporal expression of the cystatin-related epididymal spermatogenic (Cres) gene in mouse testis and epididymis during postnatal development. METHODS The QuantiGene assay and indirect immunofluorescence technique were used to examine the Cres mRNA and Cres protein level in mouse testis and epididymis on postnatal days 14, 20, 22, 28, 35, 49, 70 and 420. RE...

Differentiation of Mouse Embryonic Stem Cell into Insulin-Secreting Cell

Purpose: Differentiation of mouse embryonic stem cells into Insulin secreting endocrine cells. Materials and Methods: In this study, Royan B1 mouse embryonic stem cell (derived from C57BL/6 mouse) were used. In directed differentiation method, embryonicstem cells after embryoid bodies formation were differentiated into insulin secreting cells. Nestin positive cells were obtained after culture ...

P-92: Retinoic Acid and STO Co-Culture System Enhance Mouse Embryonic Stem Cell

Id4 Marks Spermatogonial Stem Cells in the Mouse Testis

Mammalian spermatogenesis is a classic adult stems cell-dependent process, supported by the self-renewal and differentiation of spermatogonial stem cells (SSCs). However, the identification of SSCs and elucidation of their behaviors in undisturbed testis has long been a big challenge. Here, we generated a knock-in mouse model, Id4-2A-CreERT2-2A-tdTomato, which allowed us to mark Id4-expressing ...